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cas9 as a versatile tool for engineering biology

rna-programmed genome editing in human cells

prashant mali

mali science

targeted genome engineering in human cells with the cas9 rna-guided endonuclease

multiplex genome engineering using crispr/cas systems

m. jinek et al., science 337, 816–821 (2012).

human cas9




Critical Review of “RNA-guided Human Genome Engineering via Cas9”. This paper examines a study which used the CRISPR-Cas system in order to essentially develop a “library” of sequences on genes that can be targeted for genetic engineering. The CRISPR-Cas system is the bacterial/archaeal equivalent of an
Abstract Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that..
CRISPR consists of two components: a “guide” RNA. (gRNA) and a non-specific CRISPR-associated endonuclease (Cas9). • The gRNA is a short synthetic RNA composed of a. “scaffold” sequence necessary for Cas9-binding and a user-defined 20 nucleotide “spacer” or. “targeting” sequence which targets the genomic.
We believe tools and techniques based on Cas9, a single unifying factor capable of colocalizing RNA, DNA and protein, will grant unprecedented control over cellular organization, regulation Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.
15 Nov 2016 RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably . Using tailored 20-nt target recognition sequences in a crRNA array, double deletion and/or multiplexed modifying took place in human genomes in one step.
Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033. Epub 2013 Jan 3. RNA-guided human genome engineering via Cas9. Mali P(1), Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Author information: (1)Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
5 October 2012; accepted 12 December 2012. Published online 3 January 2013;. 10.1126/science.1231143. RNA-Guided Human Genome. Engineering via Cas9. Prashant Mali,1* Luhan Yang,1,3* Kevin M. Esvelt,2 John Aach,1 Marc Guell,1 James E. DiCarlo,4. Julie E. Norville,1 George M. Church1,2†. Bacteria and
3 Jan 2013 Bacteria and archaea have evolved adaptive immune defenses termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems that use short RNA to direct degradation of foreign nucleic acids. Here, we engineer the type II bacterial CRISPR system to function
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM Science. 2013 Jan 3. PubMed Journal. Plasmids from Article. Showing 1 to 10 of 10 entries. Show. 10, 25, 50, 100, All. entries. Search table: Previous; 1; Next. ID, Plasmid. ID. Plasmid
22 Sep 2013 demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences
     

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